2 September 2019

How to determine CRISPR gene editing success in 2 weeks

Imagine CRISPR – but more efficient, reliable and flexible. SNIPER works in synergy with the CRISPR-Cas9 system to fulfill even the most challenging gene editing projects – including biallelic modifications, single base-pair changes and large DNA insertions. Read on to discover four examples of CRISPR-SNIPER in action.

What is SNIPER?

SNIPERis a highly sensitive screening platform that combines digital PCR technology with a checkerboard of culture conditions. In combination with CRISPR, SNIPER provides an advanced gene editing system that is 30X more efficient than conventional techniques*, making it easier to detect cells containing your desired mutation. CRISPR-SNIPER allows evaluation of gene editing efficiency under multiple test conditions and carries the freedom to operate for research use – drug screening protocols included.

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Why is CRISPR-SNIPER Important?

Thanks to its enhanced sensitivity, CRISPR-SNIPER can achieve genetic modifications which are challenging using CRISPR alone. It has the power to insert and detect single base-pair changes, functional gene insertions, and biallelic modifications in all types of cell-lines including iPSCs – making it the ideal system for creating custom disease models.

As SNIPER accelerates the initial screening process, the feasibility of your project can be confirmed within just two weeks. Not only is this platform the fastest screening technology available on the gene editing market, but it has the power to eliminate impossible modifications at an early stage – saving you time, effort and money.

READ: 5 Ways SNIPER can enhance your CRISPR gene editing →


Choose CRISPR-SNIPER technology, and you will harness the power to:

  • Solve challenging gene editing projects
  • Avoid follow-through of unfeasible modifications
  • Accelerate the screening process
  • Make multiplex gene editing a reality

 

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Four Examples of CRISPR-SNIPER in Action

1. Knock-out multiple genes in a single experiment

By optimizing multiple guide RNAs, CRISPR-SNIPER can achieve multiplex gene knock-out or knock-in. Up to five distinct genes in a single experiment can be edited, thereby negating the effects of extended passaging and allowing the modification of several pathway components at once. This not only extends the life of your cells, but also increases the reliability of your results.

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2. Insert large gene fragments

Where gene editing efficacy normally decreases when your DNA insert exceeds 2 kbp, CRISPR-SNIPER allows the insertion and detection of gene fragments up to 7 kbp in size. Such an advanced gene editing system permits the insertion of reporter genes which can be used to track gene expression.

For example, fluorescence markers can be inserted to monitor the development of your iPSCs or allow the isolation of specific cell types.  

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3. Generate biallelic modifications

Your disease of interest may display a variable phenotype depending on whether the disease-causing mutation is homozygous or heterozygous. However, it can be challenging to create heterozygous mutants when your gene of interest shares homology with another gene.

The accelerated screening power and high fidelity digital primer/probe component of SNIPER allow the CRISPR-SNIPER system to distinguish between DNA sequences that are similar, enabling the creation of both heterozygous and homozygous mutants.

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4. Increase gene editing accuracy

One of the most difficult genome modifications to achieve are single nucleotide polymorphisms (SNPs). Single base-pair changes are responsible for many monogenic diseases but can be tricky to create at a high frequency with conventional gene editing protocols. With CRISPR-SNIPER’s editing and enhanced screening efficiency, these precise genetic changes can be created with high reproduciblity.

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Experts in Gene Editing 

SNIPER technology works in synergy with the gold-standard CRISPR-Cas9 system to provide the most advanced gene editing system on the market. CRISPR-SNIPER combines the stem cell expertise of REPROCELL with the genome editing knowledge of GenAhead Bio to provide clinically relevant disease models.

Although there are many companies that offer gene editing and iPSC technologies, there are few that combine both services with a high success rate. If you are interested in finding out more about the science behind SNIPER, contact one of our scientists today.

 

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Find out more

Explore our free online resources and discover more about the world’s faster gene editing screening service.


SNIPER is the acronym for Specification of Newly Integrated Position and Exclusion of Random-Integration

*For SNP modification, SNIPER screening detects positive clones at a 30X higher frequency (30%) compared with conventional screening (1%)

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