How to Characterize Stem Cells using Immunocytochemistry

    Planning an immunocytochemistry (ICC) project can be tricky as it requires careful selection of compatible antibodies, fluorochromes, and blocking reagents. In this article our scientists have provided a step-by-step guide for single staining of iPSCs, plus trouble-shooting tips you can use to optimize your results. 

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    How SynFire® Technology can benefit your research

    In celebration of our distributorship with NeuCyte, REPROCELL catches up with NeuCyte’s Head of Neuroscience, Dr Daniel Haag, to discuss the benefits of SynFire technology. Read on to find out the science behind this technology, and how using human neural models can benefit your drug discovery and toxicology research.

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    How to mass culture iPSCs in suspension

    Induced pluripotent stem cells (iPSCs) have the potential to revolutionize the fields of drug discovery, disease modelling, personalized medicine and regenerative medicine [1]. iPSCs have the capacity for continuous self-renewal and differentiation into several different cell types. At the same time, they also do not face many of the ethical challenges faced by human embryonic stem cells [2].

    In order to fully realize the potential of iPSCs for many of the above applications, there is a requirement to generate a vast number of cells; hence, an effective method of mass culture of iPSCs is highly sought after by iPSC researchers.

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    Improve your gene-editing success with CRISPR-SNIPER technology

    The discovery of both CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and associated proteins, the Cas nucleases, has revolutionized the field of gene-editing.  Characterization of the role of the CRISPR-Cas system in pathogen detection, the immune response, and DNA repair mechanisms, has enabled this naturally occurring biological system to be harnessed and developed as a valuable research tool.

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    5 factors to consider when planning a stem cell reprogramming project

    When you are planning a project involving stem cell reprogramming, there are several factors to consider when choosing a reprogramming technology and a service provider. This post will highlight 5 questions you may want to answer before choosing a service provider or reprogramming technology.

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    Pluripotent Potential for Clinical Application

    Since their development in the mid-2000s, the versatile nature of induced Pluripotent Stem Cells (iPSCs) has unlocked the potential of curative approaches instead of symptom-reactive treatments. In particular, fields which deal with genetic disorders and regenerative therapies would benefit from this. The advancement of iPSC technology compliments the advent of personalized medicine, allowing for a future where individuals could be treated using autologous iPSCs.

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    Stem Cells for Autism

    In this post, we highlight recent advancements in stem cells as treatment and study of autism to commemorate World Autism Awareness Day (2 April 2019).

    Autism spectrum disorder (ASD) is a complex developmental disability that impairs the ability to communicate and relate to others. Affecting 1% of the world population, it is the most commonly diagnosed childhood developmental disorder. In 2018, Centers for Disease Control (CDC) reported that rates of autism had increased to an estimated 1 in 59 children, up from 1 in 88 just six years ago.

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    Spotlight: StemRNA Neuro iPSCs – Human Brain Neurons for Functional Assays

    Human induced pluripotent stem cell (iPSCs) derived  neural models to study brain disease provide an unlimited resource for disease modelling as well as being a tool for drug screening for effective therapies. The limited access to viable patient neurons from brain tissue itself generates the need for functional and reproducible human neuron cell models. Such cells are now increasingly used for drug development studies as well as supporting research into mechanisms and pathways of various neurological diseases.

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    Using human iPSCs as an in vitro model for regenerative medicine, drug screening and disease modelling

    In translational medicine, research using primary patient samples is advantageous over cell lines as the expression of the protein under investigation is regulated by native elements in primary samples.

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    Tissue Sourcing for the Development of iPSC-derived Disease Models

    Human induced pluripotent stem cells (iPSCs)-based models are an ideal resource for studying disease mechanisms in vitro at the cellular level[1], screening potential new therapeutics[2], and investigating the propensity and mechanism for the development of toxic side effects caused by drug treatment[3]. Such iPSC-based models enable studies to be performed under defined experimental conditions and in a reproducible manner.

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