How to improve your 3D Cell Culture using Small-scale Perfusion Systems

Three-dimensional (3D) in vitro culture is now a recognized technique to improve the performance and physiological relevance of immortalized and primary cells. 3D culture also increases proliferation rates in some cell types (e.g. mesenchymal stem cells[1], osteosarcoma cells[2], HUVECs[3], which puts extra demands on nutrient supply and waste removal. This issue can be addressed by the use of bioreactors, which circulate a large quantity of medium over or around the 3D culture and further improve cell proliferation and specific protein expression beyond that seen for static 3D cultures (e.g. dermal fibroblasts[4], cardiac cells[5], marrow stromal cells[6], osteogenic cells[7].

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3D epithelial barrier assays in vitro: How to get the best balance between practicability and physiological relevance

Barrier assays are an important tool for in vitro drug development and drug safety testing, e.g. assessing the absorption of a drug candidate through the intestinal wall or the corrosion of chemical compounds on skin.

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Prof Stefan Przyborski to chair the SMi Group’s 3D Cell Culture 2017 Conference (22-23 Feb)

The SMi Group’s 3D Cell Culture 2017 Conference (22-23 Feb) will be chaired by REPROCELL Europe’s chief scientific officer (CSO), Professor Stefan Przyborski. He will also be giving the first lecture in the conference.

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REPROCELL Europe wins CV Technology Innovator Awards 2016 for Alvetex

REPROCELL Europe has won the Corporate Vision Technology Innovation Award 2016 for Alvetex technology for 3D cell culture.

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Prof Stefan Przyborski speaking at BBS 2016 Biennial Meeting

ReproCELL Europe Ltd’s Chief Scientific Officer Prof Stefan Przyborski is giving a public lecture on “Scaffold Cell Culture Technology for Generation of In Vivo-like Tissue Models” at the British Biophysical Society 2016 Biennial Meeting 6th-8th July, University of Liverpool, Liverpool, UK.

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REPROCELL Europe’s (formerly Reinnervate’s) CSO Prof Stefan Przyborski to give an Alvetex Seminar at the University of Oxford, 24 May 2016

Professor Stefan Przyborski, Chief Scientific Officer of REPROCELL Europe (formerly Reinnervate), is to give a seminar on Alvetex next week, 24th May 2016.

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Reinnervate Ltd wins Corporate Vision’s 2015 Technology Innovator Award for Alvetex

We are pleased to announce that Reinnervate Ltd has been awarded Corporate Vision’s 2015 Technology Innovator Award for Alvetex 3D cell culture. Alvetex has been awarded the title, “Most Advanced 3D Cell Technology.”

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Alvetex in Space

While the first British ESA astronaut Tim Peake is currently on the International Space Station (ISS) and is about to make his first spacewalk, now is a good time to mention again that Reinnervate’s flagship product, Alvetex® Scaffold, has been used as part of a BBSRC-funded project on the ISS in recent months.

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Alvetex® 3D cell culture platform reaches new heights with project aboard the International Space Station (ISS).

Sedgefield, UK. April 2015 – Market leading 3D cell culture company Reinnervate Ltd, part of the ReproCELL group, today announced that a rocket carrying its Alvetex® Scaffold technology blasted into Space this week. Alvetex will be used in experiments on board the International Space Station (ISS).

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New paper demonstrates formation of spontaneously active neuronal networks by stem cell derived neurons in 3D Alvetex Scaffold culture system

A recently published paper by Imogen Smith et al. demonstrates the use of the Alvetex platform to culture 3D differentiated human neural stem cells. The cells formed spontaneously active, functional neuronal networks which were not seen in an otherwise comparable 2D culture system. The 3D neural networks responded reproducibly to pharmacological treatments revealing functional glutamatergic synapses. Further imaging analysis revealed a neuronal and glial population, where markers of maturity were apparent in the former. Microrarray analysis of the cultures of the 3D and 2D neuronal cultures showed substantial differences in the gene expression profile of genes coding for neuronal function, extracellular matrix and cytoskeleton.

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