How to improve your 3D Cell Culture using Small-scale Perfusion Systems

    Three-dimensional (3D) in vitro culture is now a recognized technique to improve the performance and physiological relevance of immortalized and primary cells. 3D culture also increases proliferation rates in some cell types (e.g. mesenchymal stem cells[1], osteosarcoma cells[2], HUVECs[3], which puts extra demands on nutrient supply and waste removal. This issue can be addressed by the use of bioreactors, which circulate a large quantity of medium over or around the 3D culture and further improve cell proliferation and specific protein expression beyond that seen for static 3D cultures (e.g. dermal fibroblasts[4], cardiac cells[5], marrow stromal cells[6], osteogenic cells[7].

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    Inserts or Plates: Which format for your 3D cell culture

     

    3D cell culture. You've heard about it, you've read about it. You're probably daunted by the plethora of techniques available out there, but you want to join the growing number of scientists publishing in the 3D field or you hope it will make your drug discovery pipeline more effective.

    But how should you do it? What's the best for your cells?

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    Cost Effective, Lab-scale iPS Cell Spheroid Culture

    Many stem cell projects expand to a point where standard culture methods—plates, dishes, flasks, etc.—no longer yield sufficient cells to meet the growing needs for differentiation and regenerative medicine. At this point, larger scale options are required. Traditional bioreactors provide large volume cell culture of liters to hundreds of liters, but they are expensive to buy and expensive to operate. New small scale bioreactors fill the niche between these two extremes perfectly, providing increased cell production over plates or dishes, while being cost-effective to buy and operate.

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