6 February 2019

Tissue Sourcing for the Development of iPSC-derived Disease Models

Dr Sarah Eminli-Meissner

By Dr Sarah Eminli-Meissner

Head of Business – Stem Cells
and Global Director, Commercial R&D

iPSCs, primary cells, Stem Cells, Human Tissues, iPSC Reprogramming  

Human induced pluripotent stem cells (iPSCs)-based models are an ideal resource for studying disease mechanisms in vitro at the cellular level[1], screening potential new therapeutics[2], and investigating the propensity and mechanism for the development of toxic side effects caused by drug treatment[3]. Such iPSC-based models enable studies to be performed under defined experimental conditions and in a reproducible manner.

iPSC-based disease models typically involve multiple steps: obtaining primary target cells, generating iPSCs, differentiating the iPSCs into the disease cell type, culturing the differentiated cells under specialized conditions to develop the disease phenotype in vitro, treating the cell-model with test agents and evaluating changes in phenotype (Figure 1).

 

Typical pathway for developing iPSC-based disease models

Figure 1. Typical pathway for developing iPSC-based disease models

 

One of the main challenges in developing iPSC-based disease models is obtaining the appropriate human tissue to initiate the process. Tissues from individuals with a particular disease allow access to cellular changes that are found in that disease state, and include all of the genotypic changes that underlie a disorder. Whether studying cellular mechanisms of certain diseases or screening for potential new drugs for therapeutic benefit, the cells used in the in vitro model should reflect the genotype and phenotype of the diseased cells in vivo which can be challenging depending on the disorder.

 

How can I access diseased tissues and generate an iPSC-based disease model?

The most straight-forward way to obtain relevant cells to initiate a disease model is to target patients with the disease of interest and obtain a source material, such as blood, urine or skin, from which to isolate primary cells to reprogram (Figure 1). This approach is advantageous in that all of the genetic changes associated with a disease phenotype are incorporated within the primary cell and will therefore be included in the derived disease model. However, disease-affected tissue can be often be difficult to obtain, especially for rarer conditions and disorders.

Another strategy is to introduce disease-causing genetic abnormalities into healthy iPSC, through gene-editing technologies such as CRISPR-Cas9. This has the advantage that genetic changes can be introduced and manipulated to assess disease mechanisms and treatment responses, and, through the use of healthy donor tissue, negates the need to obtain tissue from disease affected individuals. However, introducing and characterizing genetic modifications is an expensive and time-consuming process, and complex diseases involving multiple mutations can be difficult to model by this approach.

 

How can REPROCELL help you generate an iPSC-based disease model?

Through our extensive global Tissue Network , REPROCELL can source a variety of diseased and healthy tissue samples (for example skin, blood or urine cells) for use with REPROCELL reprogramming technologies to generate iPSCs on behalf of our clients. Tissues and cells that we source are fully anonymized and consented for use in all types of iPSC-based projects, and come with valuable donor demographic information. We can also perform molecular characterization of tissue or primary cells to provide further genetic information or confirm disease-relevant mutations.

Table 1 shows a few examples of disease areas available through our Tissue Network. Following recovery of tissue, we can source and ship directly to you, isolate the primary target cells and deliver to you, or we can directly carry the primary cells into our reprogramming protocol using our state-of-the-art StemRNA 3rd Gen Reprogramming technology state-of-the-art StemRNA 3rd Gen Reprogramming technology and deliver donor-derived iPSC ready for use (Figure 2). As our network is constantly expanding, please check with us to find out if we can source your target cell or tissue.

 

Cells can be shipped at multiple stages

Figure 2. Cells can be shipped at multiple stages

 

Table 1: Examples of Tissue Network Disease Area

Disease Group Example Diseases
Retinal Eye Disease • Retinitis Pigmentosa
• Macular Degeneration
• Leber’s Congenital Amaurosis
Metabolic Disorders • Gaucher’s Disease
• Glycogen Storage 1a disease
Neurological Disorders
and Dystrophies
• Parkinson’s Disease
• Alzheimer’s Disease
• Frontal Temporal Disorder
• Huntington’s Chorea
• Amyotrophic Lateral Sclerosis

 

 To find out more about the REPROCELL Tissue Network, Reprogramming and Molecular Services, please contact us.

 

REPROCELL-Services-02

 

References

  1. Sarkar et al. Efficient generation of CA3 neurons from human pluripotent stem cells enables modeling of hippocampal connectivity in vitro. Cell Stem Cell 22:684 (2018)
  2. Czerniecki, et al. High-throughput screening enhances kidney organoid differentiation from human pluripotent stem cells and enables automated multidimensional phenotyping. Cell Stem Cell 22:929 (2018)
  3. Holmgren et al. Expression profiling of human pluripotent stem cell-derived cardiomyocytes exposed to doxorubicin – Integration and visualization of multi-omics data. Tox Sci 163: 182 (2018)

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