Author: Frederique Tholozan

    Frederique Tholozan, Ph.D. Business Development Manager (EMEA), REPROCELL

    How to improve your 3D Cell Culture using Small-scale Perfusion Systems

    Three-dimensional (3D) in vitro culture is now a recognized technique to improve the performance and physiological relevance of immortalized and primary cells. 3D culture also increases proliferation rates in some cell types (e.g. mesenchymal stem cells[1], osteosarcoma cells[2], HUVECs[3], which puts extra demands on nutrient supply and waste removal. This issue can be addressed by the use of bioreactors, which circulate a large quantity of medium over or around the 3D culture and further improve cell proliferation and specific protein expression beyond that seen for static 3D cultures (e.g. dermal fibroblasts[4], cardiac cells[5], marrow stromal cells[6], osteogenic cells[7].

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    3D epithelial barrier assays in vitro: How to get the best balance between practicability and physiological relevance

    Barrier assays are an important tool for in vitro drug development and drug safety testing, e.g. assessing the absorption of a drug candidate through the intestinal wall or the corrosion of chemical compounds on skin.

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    Inserts or Plates: Which format for your 3D cell culture

     

    3D cell culture. You've heard about it, you've read about it. You're probably daunted by the plethora of techniques available out there, but you want to join the growing number of scientists publishing in the 3D field or you hope it will make your drug discovery pipeline more effective.

    But how should you do it? What's the best for your cells?

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